AID Carbapenemase PCR kit – detection of the major carbapenem resistances as well as the colistin resistance gene
- Developed for an initial screening of the most frequent carbapenemases with one single test
- Control bands on every strip shows correct DNA isolation, amplification and hybridization
- Suited for automated systems
- Results within 4 hours
- CE IVD
The emergence and spread of carbapenem-hydrolyzing β-lactamases among Gram-negative bacteria over the past decade represent a serious challenge in the hospital environment. This situation is particularly concerning because these enzymes hydrolyze nearly all β-lactam antibiotics and are often resistant to currently available β-lactamase inhibitors.
Enterobacterales exhibit a wide variety of carbapenemase genes. These are widely disseminated, particularly in Escherichia coli and Klebsiella pneumoniae, but can also be detected in other species such as Pseudomonas aeruginosa and Acinetobacter baumannii.
The globally most important carbapenemases include Klebsiella pneumoniae carbapenemase (KPC), Verona integron-encoded metallo-β-lactamase (VIM), New Delhi metallo-β-lactamase (NDM), and OXA-48. In Germany, OXA-48 is the most prevalent carbapenemase, followed by KPC and VIM-1. In addition, an increasing detection of NDM-1 has been observed in recent years.
Bacterial strains that have developed resistance to the last-resort antibiotic colistin, mediated by the mcr-1 resistance gene, have been increasingly reported for several years in Germany and other European countries.
